FAECAL GLUCOCORTICOID MEASUREMENT AS INDICATOR STRESS IN WILD CRESTED MACAQUES (MACACA NIGRA): THE IMPORTANCE OF VALIDATION AND SAMPLE PROCESSING TECHNIQUES

FAECAL GLUCOCORTICOID MEASUREMENT AS INDICATOR STRESS IN WILD CRESTED MACAQUES (MACACA NIGRA): THE IMPORTANCE OF VALIDATION AND SAMPLE PROCESSING TECHNIQUES

Monitoring of physiological stress in wild and/or endangered animals living in their natural habitat can be generated via measuring faecal glucocorticoid metabolites (FGCMs) through enzyme immunoassay technique. However, a careful validation of each enzyme immunoassays (EIAs) and methodological issues such as samples preservation, and extraction procedures must be a significant concern and validated because  it may influence FGCMs levels. In this present study, the aim was to carry out a biological validation to find reliable EIAs that could be used for non-invasive measurement of glucocorticoid levels in crested macaques. Moreover, we compared three different techniques of preservation: lyophilisation, oven drying and field extraction and three different solvents for extraction: 80% methanol, 90% pure ethanol and 90% commercial alcohol (isopropyl alcohol) to find the simple and practical techniques for sample processing. For hormone analysis, we colIected 189 faecal samples from wild crested macaques living in the Tangkoko-Batuangus Nature Reserve, North Sulawesi, Indonesia. The results show that biological validation can be used to validate measurement of faecal glucocorticoid which 3α,11β-dihydroxy-etiocholanolone was the most reliable EIAsfor this species. Different preservation and solvent extraction were not significantly influenced levels of FGCMs (p>0.05). In conclusion, Our study highlights the importance of biological validation of FGCMassays and presents practical techniques for the non-invasive monitoring of physiological stress in crested macaques.

Gholib, .; Study Program of Reproductive Biology, Faculty of Veterinary Medicine, Bogor Agricultural University

Nugraha, Taufiq P.

Agil, Muhammad

Supriatna, Iman

Purwantara, Bambang

Engelhardt, Antje; Research Group of Primate Sexual Selection,German Primate Center, Leibniz Institute for Primate Research, Kellnerweg 4, 37077 Goettingen, Germany

Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter; Vol 4, No 2 (2014): Life Sciences; 167-172

2089-208X

Syiah Kuala University

2014-12-30

Copyright (c) 2015 Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter

http://jurnal.unsyiah.ac.id/AICS-SciEng/article/view/2556/2415

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