SiRNA Mediated Gene Silencing: A Mini Review

SiRNA Mediated Gene Silencing: A Mini Review

Abstract - RNA interference (RNAi) technology has become a novel tool for silencing gene expression in cells or organisms. RNA interference is the process that double-stranded RNA induces the homology-dependent degradation of cognate mRNA mediated by 21-23 nucleotide short interfering RNA (siRNA). RNA interference is a powerful mechanism of gene silencing that underlies many aspects of eukaryotic biology. On the molecular level, RNAi is mediated by a family of ribonucleoprotein (RNP) complexes called RNA-Induced Silencing Complexes (RISCs), which can be programmed to target virtually any nucleic acid sequence for silencing. The ability of RISC to locate target RNAs been co-opted by evolution many times to generate a broad spectrum of gene silencing pathways. The study about the Silencing of gene expression by siRNA is rapidly becoming a powerful tool for genetic analysis and represents a potential strategy for therapeutic product development. In this study, the applications of siRNA expressing recombinant adenovirus system in plants, animals and in cancer gene therapy are given importance with its modifications

Joseph, Baby; Interdisciplinary Research Center, Malankara Catholic College, Mariagiri, Kaliakkavilai - 629153

S.U, Ajisha; Interdisciplinary Research Center, Malankara Catholic College, Mariagiri, Kaliakkavilai - 629153

M.V, Jeevitha; Interdisciplinary Research Center, Malankara Catholic College, Mariagiri, Kaliakkavilai - 629153

Aceh International Journal of Science and Technology; Vol 1, No 3: September - December 2012

2088-9860

Graduate School of Syiah Kuala University

2012-12-01

http://jurnal.unsyiah.ac.id/AIJST/article/view/140/133

application/pdf

eng

info:eu-repo/semantics/article

info:eu-repo/semantics/publishedVersion

Peer-reviewed Article

http://jurnal.unsyiah.ac.id/AIJST/article/view/140

10.13170/aijst.1.3.140