Purifikasi Imunoglobulin Yolk Pada Ayam yang Divaksin terhadap Ekskretori/Sekretori Stadium L3 Ascaridia galli

Purifikasi Imunoglobulin Yolk Pada Ayam yang Divaksin terhadap Ekskretori/Sekretori Stadium L3 Ascaridia galli

ascaridia galli; excretory; secretory antigen; yolk Immunoglobulin

Purification yolk immunoglobulin of hens vaccinated against excretory/secretory Ascaridia galli L3 larvae stageABSTRACT. The main immunoglobulin fraction of poultry is called IgY, in order to distinguish it from the mammalian IgG. This article focus on purification yolk immunoglobulin of hens vaccinated against excretory/secretory Ascaridia galli larvae to obtained purity IgY. Active vaccinations with excretory/secretory antigen were applied intra muscularly of chickens with an initial dose of 80 μg. The vaccinations were repeated three times with dose of each 60 μg with an interval of one week. The first vaccinations were excretory/secretory antigen mixed with Fruend Adjuvant Complete and subsequently mixed with Freund Adjuvant Incomplete. Antibody response in yolk was detected at weekly intervals by agar gel precipitation test (AGPT). The chicken’s eggs were collected from 49 day after vaccinations. IgY was extracted from egg yolks by means of ammonium sulphate and purified using fast performance liquid chromatography (FPLC). The purity of anti-ekscretory/secretory IgY protein was determined by Bradford method (λ = 280 nm). The result showed that antibody in yolk was begun detect with AGPT at four weeks after vaccination. IgY concentration after purification was 0,875 ± 0.251 mg/ml. This study has shown that the product released in vitro by L3 stage A. galli is capable of stimulating humoral immunity by mean of producing antibody through yolk as biologic manufacturer could be a good choice.

Darmawi, Darmawi

Balqis, Ummu

Tiuria, Risa

Hambal, Muhammad

Samadi, Samadi

Jurnal Agripet; Vol 10, No 2 (2010): Volume 10, No. 2, Oktober 2010; 9-15

2460-4534

1411-4623

Agricultural Faculty

2010-10-01

http://jurnal.unsyiah.ac.id/agripet/article/view/638/546

application/pdf

eng

info:eu-repo/semantics/article

info:eu-repo/semantics/publishedVersion

Peer-reviewed Article

http://jurnal.unsyiah.ac.id/agripet/article/view/638

10.17969/agripet.v10i2.638