Dublin Core
Title
DETECTION OF SAG1 AND BAG1 Toxoplasma gondii DNA PROBES LABELLED WITH DIGOXIGENIN-11-dUTP
Description
The objective of this research was to detect a minimum concentration of the probes that could be used for dot blot hybridization analysis. The method required labeled DNA probes. In this study a non-radioactive label of Digoxigenin-11-dUTP was used for labeling the Sag1 and the Bag1 of Toxoplasma gondii DNA probe. Labeling method for the probes was done according to the random primed labeling technique. The result showed that 0.67 pg/µl Sag1 probe and 0.58 pg/µl Bag1 probe could be detected by anti-Dig-antibody. It could be concluded that 0.67 pg/µl Sag1 probe and 0.58 pg/µl Bag1 probe could be used to diagnose toxoplasmosis by dot blot hybridization method.
Creator
Apsari, Ida Ayu Pasti
Artama, Wayan Tunas
s, Sumartono
Damriyasa, I Made
Source
Jurnal Kedokteran Hewan - Indonesian Journal of Veterinary Sciences; Vol 7, No 1 (2013): J. Ked. Hewan
Jurnal Kedokteran Hewan; Vol 7, No 1 (2013): J. Ked. Hewan
2502-5600
1978-225X
Publisher
Syiah Kuala University
Date
2013-03-01
Contributor
Rights
Copyright (c) 2016 by author and J. Ked. Hewan
http://creativecommons.org/licenses/by-sa/4.0
Relation
http://jurnal.unsyiah.ac.id/JKH/article/view/579/489
Format
application/pdf
Language
eng
Type
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
Identifier
http://jurnal.unsyiah.ac.id/JKH/article/view/579
10.21157/j.ked.hewan.v7i1.579