POTENSI BAKTERI ENDOFIT PADA TANAMAN PALA (MYRISTICA FRAGRANS) SEBAGAI AGEN ANTAGONIS UNTUK MENGENDALIKAN PENYAKIT MATI RANTING

POTENSI BAKTERI ENDOFIT PADA TANAMAN PALA (MYRISTICA FRAGRANS) SEBAGAI AGEN ANTAGONIS UNTUK MENGENDALIKAN PENYAKIT MATI RANTING

ABSTRACTThis study aims to determine the ability of endophytic bacteria from nutmeg plant in suppressing the development of dead twig disease.This research was conducted in the laboratory plant disease, department of agroteknology, faculty of Agriculture Syiah Kuala University Darussalam BandaAceh , which started on April 10 until 30 October, 2014.This study used a descriptive method, for the phase isolation of endophytic bacteria and hipersensitif test. While the phase in vitro test used experimental design completely randomized design (CRD) non factorial with 3 replication. The parameters observed was percentage inhibition of pathogenic fungi colonies.The results were obtained 17 isolates, but result of hypersensitivity test only 12 isolates classified as endophytic bacteria. 12 isolates of endophytic bacteria were tested in vitro with fungal pathogens CP1 to determine the inhibition produced by the endophytic bacterial isolates, and the results were 12 isolates of endophytic bacteria can inhibit the growth of pathogenic fungi CP1 with different percentage inhibition. Isolates the PS-S AG2(1) is able to inhibit the growth of pathogenic fungi CP1 with the highest percentage inhibition at the age of 4 DAI by 86.61%. so isolates PS-S AG2(1) is potentially be used as an antagonist agent.Key wors : nutmeg, bacteria endophitic, hypersensitivity test, in vitro test, DAI (day after isolation)

Vinny Pratiwi

http://etd.unsyiah.ac.id//index.php?p=show_detail&id=13253