Nonporous Chitosan/Collagen Scaffold for Skin Tissue Engineering

Nonporous Chitosan/Collagen Scaffold for Skin Tissue Engineering

This study aimed to determine film characteristics of chitosan/collagen scaffold for tissue engineering applications. Scaffold prepared using freeze drying method. Surface structure and biological testing chitosan/collagen scaffold crosslinking reagent addition Glutaraldehide studied using Scanning Electron Microscopy test (SEM) and Microscope inverted. Variations in the ratio of chitosan/collagen (10:0, 8:2, 7:3, 6:4, 5:5 4:6, 3:7, 2:8, 0:10), and treated with a crosslinking reagent 0.25% of Glutaraldehyde (GA) of the total weight of the polymer. The next process dissolving and mixing, followed by printing in glass moulds (7,5x7,5) with a thickness of 5 mm. This was followed by the freezing and drying with a freeze dryer. Scaffold chitosan/collagen ratio of 80:20 and a concentration of 0.25% GA showed growth of human skin fibroblast cells most and nonporous surface structure. This study is part of a study of the processing of chitosan/collagen scaffold for applications in tissue engineering

Suryati, .

Agusnar, Harry

Gea, Saharman

Ilyas, Syafruddin

Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter; Vol 2, No 2 (2012): Engineering

2089-208X

Syiah Kuala University

2012-12-15

http://jurnal.unsyiah.ac.id/AICS-SciEng/article/view/1863/1759

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eng

info:eu-repo/semantics/article

info:eu-repo/semantics/publishedVersion

Peer-reviewed Article

http://jurnal.unsyiah.ac.id/AICS-SciEng/article/view/1863