Effect of Inoculum Age, Carbon and Nitrogen Sources on the Production of Lipase by Candida Cylindracea 2031 in Batch Fermentation

Effect of Inoculum Age, Carbon and Nitrogen Sources on the Production of Lipase by Candida Cylindracea 2031 in Batch Fermentation

lipase production; candida cylindracea; palm oil; stirred bioreactor

Production of extracellular lipase by Candida cylindracea DSMZ 2031  was studied in a seven liters batch bioreactor, using palm oil (PO), palmitic acid (PA), lauric acid (LA), olive oil (OO) and cooking oil (CO) as carbon source.   The effect of  carbon and nitrogen sources  were studied by measuring the lipase activity.  The maximum lipase activity was found to be 12.7 kLU on palm oil as carbon source, urea as nitrogen sources and at 36 h inoculum age. This was achieved at a temperature of 30o C, pH of 6.0, agitation speed of 500 rpm and aeration of 1vvm.

Noor, I. M.

Hasan, M.

Ramachandran, K. B.

Jurnal Rekayasa Kimia & Lingkungan; Vol 5, No 1 (2006): Jurnal Rekayasa Kimia & Lingkungan; 48-55

2356-1661

1412-5064

Chemical Engineering Department, Syiah Kuala University, Banda Aceh, Indonesia

2006-06-01

http://jurnal.unsyiah.ac.id/RKL/article/view/1086/1019

application/pdf

eng

info:eu-repo/semantics/article

info:eu-repo/semantics/publishedVersion

Peer-reviewed Article

http://jurnal.unsyiah.ac.id/RKL/article/view/1086